Autoantibodies to double-stranded (ds)DNA immunoprecipitate 18S ribosomal RNA by virtue of their interaction with ribosomal protein S1 and suppress in vitro protein synthesis

Clin Exp Immunol. 1996 Dec;106(3):504-8. doi: 10.1046/j.1365-2249.1996.d01-869.x.

Abstract

We report that four systemic lupus erythematosus (SLE) patient sera containing anti-dsDNA antibodies, three affinity-purified anti-dsDNA IgG, and a human anti-dsDNA MoAb (33.H11) immunoprecipitate 18S ribosomal RNA from DNase-treated 32P-labelled MOLT4 cell extract. This 18S RNA precipitation was inhibited completely by preincubating 33.H11 with calf thymus dsDNA or the recombinant human ribosomal protein S1, which was reported to cross-react with anti-dsDNA antibodies (J Immunol 1996; 156:1668-75). Whole IgG from three SLE sera with anti-dsDNA antibodies, 33.H11, and three affinity-purified anti-dsDNA IgG inhibited in vitro translation of globin mRNA (percent inhibition was 36-50%). This translation inhibition by anti-dsDNA antibodies was enhanced (67-79%) when the reticulocyte lysate was treated with DNase. Suppression of protein synthesis could be a pathogenic mechanism of anti-dsDNA antibodies, since it has also been shown that anti-dsDNA penetrates living cells (J Immunol 1995; 154:4857-64) in culture.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Antinuclear / immunology*
  • Antibodies, Antinuclear / pharmacology*
  • Cell Line
  • Cross Reactions / immunology
  • DNA / immunology*
  • Humans
  • Lupus Erythematosus, Systemic / immunology
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / immunology*
  • Peptide Elongation Factors / metabolism*
  • Precipitin Tests
  • Protein Biosynthesis*
  • Proteins / drug effects*
  • RNA, Ribosomal, 18S / drug effects*
  • RNA, Ribosomal, 18S / immunology
  • RNA, Ribosomal, 18S / metabolism*
  • Ribonucleoproteins / immunology
  • Ribonucleoproteins / metabolism
  • Suppressor Factors, Immunologic / immunology*

Substances

  • Antibodies, Antinuclear
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors
  • Proteins
  • RNA, Ribosomal, 18S
  • Ribonucleoproteins
  • Suppressor Factors, Immunologic
  • DNA