Abstract
A bovine RAPD profile, generated by a 10-mer primer, was analysed by sequencing the major fragments. Three of four different fragments showed homologies to previously characterized mammalian sequences. One was 61-66% identical to LINE sequences and another was 78.5% identical to a human chromosome 2 sequence tagged site. The third fragment was 93.1% identical to the human type 2 inositol 1,4,5-trisphosphate receptor gene. This fragment had counterparts in white-tailed deer and reindeer; fragments of slightly different size in these species showed high sequence similarity and the size differences were due to varying numbers of dinucleotide microsatellite repeats inside the fragment.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Artiodactyla / genetics*
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Base Sequence
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Calcium Channels / genetics
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Cattle
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Chromosomes, Human, Pair 2 / genetics
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DNA / genetics*
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DNA Primers / genetics
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Deer / genetics
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Female
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Humans
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Inositol 1,4,5-Trisphosphate Receptors
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Male
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Molecular Sequence Data
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Pedigree
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Random Amplified Polymorphic DNA Technique
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Receptors, Cytoplasmic and Nuclear / genetics
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Reindeer / genetics
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Sequence Homology, Nucleic Acid
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Sequence Tagged Sites
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Species Specificity
Substances
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Calcium Channels
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DNA Primers
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ITPR1 protein, human
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ITPR2 protein, human
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Inositol 1,4,5-Trisphosphate Receptors
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Receptors, Cytoplasmic and Nuclear
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DNA