Cloning and characterization of one member of the chalcone synthase gene family from Solanum tuberosum L

J H Jeon; H S Kim; K H Choi; Y H Joung; H Joung; S M Byun

doi:10.1271/bbb.60.1907

Cloning and characterization of one member of the chalcone synthase gene family from Solanum tuberosum L

Biosci Biotechnol Biochem. 1996 Nov;60(11):1907-10. doi: 10.1271/bbb.60.1907.

Authors

J H Jeon¹, H S Kim, K H Choi, Y H Joung, H Joung, S M Byun

Affiliation

¹ Department of Biological Science, Korea Advanced Institute of Science and Technology, Taejon, Korea. [email protected]

PMID: 8987872
DOI: 10.1271/bbb.60.1907

Abstract

We have isolated a chalcone synthase gene 2 (ST-CHS2) from potato by rapid amplification of cDNA ends by PCR. CHS2 cDNA had high homology to tomato LET-CHS2 (98%), petunia PHCHSJ (94%), potato ST-CHS1B (92%), petunia PHCHSA (92%), and LET-CHS1 (90%) at the overall 389-amino acid level. Genomic hybridization analysis indicated that CHS genes of potato comprise a family of at least six individual members.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyltransferases / genetics*
Acyltransferases / metabolism
Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
Cloning, Molecular
DNA, Complementary / biosynthesis
DNA, Complementary / genetics
In Situ Hybridization
Molecular Sequence Data
Plants / enzymology*
Plants / genetics*

Substances

DNA, Complementary
Acyltransferases
flavanone synthetase

Associated data

GENBANK/U47738