Isolation and characterization of Tula virus, a distinct serotype in the genus Hantavirus, family Bunyaviridae

J Gen Virol. 1996 Dec:77 ( Pt 12):3063-7. doi: 10.1099/0022-1317-77-12-3063.

Abstract

A Vero E6 cell culture isolate of Tula virus (TUL), a hantavirus first detected in European common voles (Microtus arvalis and M. rossiaemeridionalis) by RT-PCR was obtained after initial passaging of TUL-infected vole lung samples in laboratory-colonized M. arvalis. TUL was defined as a classical serotype by a cross-focus-reduction neutralization test (FRNT) and was also shown to be distinct from other hantaviruses by haemagglutination inhibition assay. The sequences of S, M and partial L genome segments of the isolate were determined: the S segment was 99.9% identical to the original rodent-derived sequence. Serological evidence for a previous TUL infection was obtained from the serum of a blood donor living near a TUL focus in Moravia, Czech Republic, showing at least a 16-fold higher FRNT titre to TUL as compared to Puumala or other hantaviruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Arvicolinae / virology
  • Base Sequence
  • Chlorocebus aethiops
  • Hantavirus Infections / immunology
  • Hantavirus Infections / pathology
  • Hantavirus Infections / virology*
  • Molecular Sequence Data
  • Orthohantavirus / genetics
  • Orthohantavirus / immunology
  • Orthohantavirus / isolation & purification*
  • RNA, Viral / analysis*
  • Rabbits
  • Serotyping
  • Vero Cells

Substances

  • Antibodies, Viral
  • RNA, Viral

Associated data

  • GENBANK/Z69991
  • GENBANK/Z69992
  • GENBANK/Z69993