Abstract
Cells transformed by human adenovirus 12 (Ad12) exhibit extremely low surface levels of MHC class I molecules and contain reduced levels of class I heavy chain mRNAs. We report that levels of MHC-encoded TAP1 and LMP2 mRNAs are also down-regulated in Ad12-transformed rat cells, and that transcription of rat TAP1 and LMP2 transcripts is directed from a 564 bp intergenic region which is significantly less active in Ad12-transformed cells compared to those transformed with Ad5. Our results suggest that, in common with MHC class I gene expression, TAP1 and LMP2 gene expression is reduced mainly at the level of transcription in Ad12-transformed cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 2
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ATP-Binding Cassette Transporters / genetics*
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Adenoviruses, Human / genetics*
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Animals
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Base Sequence
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Cell Line, Transformed
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Cloning, Molecular
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Cysteine Endopeptidases*
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DNA, Complementary
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Down-Regulation*
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Gene Expression
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Gene Expression Regulation*
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Humans
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Major Histocompatibility Complex*
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Molecular Sequence Data
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Oncogenes
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Promoter Regions, Genetic*
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Proteins / genetics*
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RNA, Messenger
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Rats
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Rats, Wistar
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 2
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ATP-Binding Cassette Transporters
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DNA, Complementary
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Proteins
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RNA, Messenger
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TAP1 protein, human
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Tap1 protein, rat
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LMP-2 protein
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Cysteine Endopeptidases