Characterization of rat receptor-like protein tyrosine phosphatase gamma isoforms

Biochem Biophys Res Commun. 1997 Jan 13;230(2):419-25. doi: 10.1006/bbrc.1996.5973.

Abstract

We identified four isoforms of receptor-like protein tyrosine phosphatase gamma (RPTPgamma) from rat brain by cDNA cloning. We designated these molecules RPTPgamma-A, -B, -C, and -S. RPTPgamma-A was the longest form and had the same structure as human and mouse RPTPgamma. RPTPgamma-B lacked the intracellular juxtamembrane 29 amino acids of RPTPgamma-A. RPTPgamma-C had a single phosphatase domain. RPTPgamma-S is an extracellular variant of RPTPgamma. mRNAs of the four isoforms were expressed in the brain, kidney, lung, and heart. Transfection of RPTPgamma-A and -S expression plasmids into COS7 cells resulted in the expression of membrane-bound 190-kDa proteins and secreted 120-kDa proteins, respectively. These molecules were similar to PTPzeta/RPTPbeta with regard not only to structure but also to the presence of both secretory and transmembrane forms. However, RPTPgamma isoforms were not expressed as proteoglycans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / enzymology*
  • COS Cells
  • Cell Membrane / enzymology
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • Genetic Variation
  • Humans
  • Isoenzymes / biosynthesis*
  • Isoenzymes / chemistry
  • Kidney / enzymology
  • Lung / enzymology
  • Mice
  • Molecular Sequence Data
  • Myocardium / enzymology
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / chemistry
  • Polymerase Chain Reaction
  • Protein Tyrosine Phosphatases / biosynthesis*
  • Protein Tyrosine Phosphatases / chemistry
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptor-Like Protein Tyrosine Phosphatases, Class 5
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / chemistry
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*
  • Transfection

Substances

  • DNA Primers
  • DNA, Complementary
  • Isoenzymes
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Recombinant Proteins
  • PTPRG protein, human
  • Protein Tyrosine Phosphatases
  • Ptprg protein, mouse
  • Ptprg protein, rat
  • Receptor-Like Protein Tyrosine Phosphatases, Class 5