Characterization of two new matrices for matrix-assisted laser desorption/ionization mass spectrometry

Rapid Commun Mass Spectrom. 1996;10(7):839-44. doi: 10.1002/(SICI)1097-0231(199605)10:7<839::AID-RCM579>3.0.CO;2-C.

Abstract

Three structurally related compounds, 4-hydroxy-33-methoxyphenylpyruvic acid (HMPPA), indole-3 pyruvic acid (IPA), and indole-3-glyoxylic acid have been evaluated as matrix-assisted laser desorption/ionization (MALDI) matrices. HMPPA and IPA were found to be effective matrices for MALDI-MS analysis of proteins and peptides and have been characterized in this work. HMPPA was found to be a particularly good matrix for the analysis of proteins and peptides because of its tolerance towards impurities and the resulting sensitivity in MALDI-MS experiments. Analysis of model proteins and peptides, including those in biological fluid, are demonstrated.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Glyoxylates / chemistry
  • Indoles / chemistry
  • Peptides / chemistry
  • Phenylpyruvic Acids / chemistry
  • Proteins / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

  • Glyoxylates
  • Indoles
  • Peptides
  • Phenylpyruvic Acids
  • Proteins
  • vanilpyruvic acid
  • indole-3-glyoxylic acid
  • indol-3-yl pyruvic acid