Abstract
In normal human cells, damage due to ultraviolet light is preferentially removed from active genes by nucleotide excision repair (NER) in a transcription-coupled repair (TCR) process that requires the gene products defective in Cockayne syndrome (CS). Oxidative damage, including thymine glycols, is shown to be removed by TCR in cells from normal individuals and from xeroderma pigmentosum (XP)-A, XP-F, and XP-G patients who have NER defects but not from XP-G patients who have severe CS. Thus, TCR of oxidative damage requires an XPG function distinct from its NER endonuclease activity. These results raise the possibility that defective TCR of oxidative damage contributes to the developmental defects associated with CS.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
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Retracted Publication
MeSH terms
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Cockayne Syndrome / genetics*
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Cockayne Syndrome / metabolism
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DNA / biosynthesis
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DNA Damage
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DNA Repair*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Endonucleases / chemistry
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Endonucleases / genetics*
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Endonucleases / metabolism
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Genetic Complementation Test
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Humans
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Hydrogen Peroxide / pharmacology
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Mutation
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Nuclear Proteins
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Oxidation-Reduction
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Transcription Factors
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Transcription, Genetic*
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Ultraviolet Rays
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Xeroderma Pigmentosum / genetics*
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Xeroderma Pigmentosum / metabolism
Substances
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DNA excision repair protein ERCC-5
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DNA-Binding Proteins
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Nuclear Proteins
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Transcription Factors
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DNA
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Hydrogen Peroxide
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Endonucleases