PAF and haematopoiesis: IX. Platelet-activating factor increases DNA synthesis in human bone marrow cells

J Lipid Mediat Cell Signal. 1996 Dec;15(1):1-4. doi: 10.1016/s0929-7855(96)00447-6.

Abstract

Platelet-activating factor (PAF) is present in human bone marrow leading us to investigate its effect on human bone marrow cell proliferation. While PAF (0.1 microM to 1 nM) stimulates the incorporation of [3H]thymidine by freshly isolated adherent human bone marrow cells, PAF has no effect on non adherent cells. A non-metabolizable PAF agonist is more potent than PAF to stimulate thymidine incorporation in adherent cells. The precise role of PAF in human haematopoiesis in vivo remains to be clarified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Marrow / drug effects*
  • Bone Marrow / metabolism*
  • Bone Marrow Cells
  • Cells, Cultured
  • DNA / biosynthesis*
  • Hematopoiesis / drug effects*
  • Humans
  • Platelet Activating Factor / pharmacology*
  • Thymidine / metabolism
  • Tritium

Substances

  • Platelet Activating Factor
  • Tritium
  • DNA
  • Thymidine