Sequence analysis of hepatitis GB virus C (GBV-C) isolates from 14 patients

Virus Res. 1996 Dec;46(1-2):81-8. doi: 10.1016/s0168-1702(96)01377-9.

Abstract

In 1995, a new human hepatitis virus belonging to the family Flaviviridae was described and designated hepatitis GBV-C. To investigate variations within the genome of GBV-C and to study the relationship of GBV-C to GBV-A/B or hepatitis C virus (HCV), we established a detection system using reverse transcriptase polymerase chain reaction (RT-PCR) of the putative helicase region (NS3). So far, isolates derived from 14 different GBV-C-positive sera were analyzed (GBV-C/S3-36), showing 80.1-89.4% (mean: 85%) identical nucleotides. The deduced amino acid sequences revealed 97.3% homology. Nucleotide sequences of GBV-C/S3-36 revealed about 60% identity to GBV-A as well as to HCV, but only 56% identity to GBV-B. Amino acid sequences revealed 73.4 and 68.6% similarity to GBV-A and GBV-B, respectively, but a slightly higher percentage of 78.5% to HCV sequences. Thus, according to the putative GBV-C helicase sequence, a subtyping of GBV-C into different genotypes may be necessary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Cloning, Molecular
  • Flaviviridae / genetics*
  • Flaviviridae / isolation & purification*
  • Hepatitis, Viral, Human / genetics
  • Hepatitis, Viral, Human / virology*
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Associated data

  • GENBANK/D10749
  • GENBANK/M62321
  • GENBANK/U22303
  • GENBANK/U22304
  • GENBANK/U25538
  • GENBANK/U25539
  • GENBANK/U25540
  • GENBANK/U25541
  • GENBANK/U25542
  • GENBANK/U25543
  • GENBANK/U25544
  • GENBANK/U25545
  • GENBANK/U36380
  • GENBANK/U44402
  • GENBANK/U45966
  • GENBANK/U63197
  • GENBANK/U63198
  • GENBANK/U63199
  • GENBANK/U63200
  • GENBANK/U63201
  • GENBANK/U63202
  • GENBANK/U63203
  • GENBANK/U63204
  • GENBANK/U63205
  • GENBANK/U63206
  • GENBANK/U63207
  • GENBANK/U63208
  • GENBANK/U63209
  • GENBANK/U63210