Abstract
Single chain fragment (ScFv) antiidiotypic antibodies (antilds) of a killer toxin (KT) from the yeast Pichia anomala have been produced by recombinant DNA methodology from the splenic lymphocytes of mice immunized by idiotypic vaccination with a KT-neutralizing monoclonal antibody (Mab KT4). ScFv KT-like antilds (KTIdAb) react with specific Candida albicans KT cell wall receptors (KTR) exerting a candidacidal activity in vitro could be neutralized by adsorption with Mab KT4. ScFv KTIdAb displayed an effective therapeutic activity in an experimental model of rat candidal vaginitis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Anti-Idiotypic*
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Antibodies, Monoclonal*
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Candida albicans / drug effects
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Candida albicans / immunology
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Candida albicans / physiology
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Candidiasis, Vulvovaginal / drug therapy*
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DNA, Recombinant
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Drug Design
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Female
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Genetic Engineering
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Immunotoxins / pharmacology
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Immunotoxins / therapeutic use*
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Killer Factors, Yeast
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Mice
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Microbial Sensitivity Tests
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Mycotoxins / biosynthesis
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Mycotoxins / pharmacology
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Mycotoxins / therapeutic use*
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Pichia
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Rats
Substances
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Antibodies, Anti-Idiotypic
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Antibodies, Monoclonal
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DNA, Recombinant
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Immunotoxins
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Killer Factors, Yeast
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Mycotoxins