Abstract
The small guanosine triphosphatase (GTPase) Rho is implicated in the formation of stress fibers and focal adhesions in fibroblasts stimulated by extracellular signals such as lysophosphatidic acid (LPA). Rho-kinase is activated by Rho and may mediate some biological effects of Rho. Microinjection of the catalytic domain of Rho-kinase into serum-starved Swiss 3T3 cells induced the formation of stress fibers and focal adhesions, whereas microinjection of the inactive catalytic domain, the Rho-binding domain, or the pleckstrin-homology domain inhibited the LPA-induced formation of stress fibers and focal adhesions. Thus, Rho-kinase appears to mediate signals from Rho and to induce the formation of stress fibers and focal adhesions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Actins / metabolism*
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Adenosine Triphosphate / metabolism
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Animals
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Binding Sites
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Cell Adhesion*
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Cell Line
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DNA, Complementary / genetics
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Enzyme Inhibitors / pharmacology
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GTP Phosphohydrolases / metabolism
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Intracellular Signaling Peptides and Proteins
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Lysophospholipids / pharmacology
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Mice
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Mutation
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Protein Serine-Threonine Kinases / antagonists & inhibitors
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Recombinant Fusion Proteins / metabolism
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Staurosporine / pharmacology
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rho-Associated Kinases
Substances
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Actins
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DNA, Complementary
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Enzyme Inhibitors
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Intracellular Signaling Peptides and Proteins
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Lysophospholipids
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Recombinant Fusion Proteins
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Adenosine Triphosphate
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Protein Serine-Threonine Kinases
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rho-Associated Kinases
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GTP Phosphohydrolases
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Staurosporine