Cloning of the cDNA encoding rat Presenilin-1

Gene. 1997 Feb 20;186(1):73-5. doi: 10.1016/s0378-1119(96)00683-x.

Abstract

We isolated rat presenilin-1 (PS-1; also called S182 previously) cDNA from total brain RNA by using a reverse transcription-polymerase chain reaction (RT-PCR) technique with primers homologous to the conserved sequences of human and mouse PS-1. Rat PS-1 cDNA encoded 468 amino acids (aa) and the deduced aa sequence was highly homologous to those of the human (88.4%) and mouse (92.7%). Northern blot analysis of the rat PS-1 cDNA revealed two mRNA species in rat neurotypic pheochromocytoma and glioma cell lines (PC-12 and C6, respectively) that migrated at rates corresponding to approximately 3.0 and 7.5 kb.

MeSH terms

  • Alzheimer Disease / genetics
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Blotting, Northern
  • Cells, Cultured
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Presenilin-1
  • RNA, Messenger / biosynthesis
  • Rats
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Membrane Proteins
  • PSEN1 protein, human
  • Presenilin-1
  • RNA, Messenger

Associated data

  • GENBANK/D82578