The regulated expression of cell cycle-related proteins as B-lymphocytes enter and progress through the G1 cell cycle stage following delivery of complete versus partial activation stimuli

Mol Immunol. 1996 Oct;33(14):1139-51. doi: 10.1016/s0161-5890(96)00065-x.

Abstract

Resting B-cells lack both cyclins D and E while constitutively expressing low levels of cdk4 and cdk2. B-cells receiving a complete growth stimulus express cyclin D2 by 10hr and cyclin E by 10-24hr poststimulation while increasing their protein levels of cdk4 and cdk2. B-cells receiving partial growth stimuli move into G1 without passing the G1 restriction point and transiently increase cyclin D2 mRNA levels without accumulating cyclin D2 protein. In the absence of cyclin D2 accumulation, cdk4 is not activated, and cyclin E is not expressed. These results suggest that signals responsible for moving B-cells through the G1 restriction point impact at the level of cyclin D2 protein accumulation. The possible implications of these results are discussed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism*
  • Cell Cycle / immunology*
  • Cyclin-Dependent Kinases / biosynthesis*
  • Cyclin-Dependent Kinases / drug effects*
  • Cyclin-Dependent Kinases / genetics
  • Cyclins / biosynthesis*
  • Cyclins / drug effects*
  • Cyclins / genetics
  • Female
  • G1 Phase / immunology*
  • Gene Expression Regulation / immunology
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred DBA
  • Mitogens / pharmacology
  • RNA, Messenger / analysis

Substances

  • Cyclins
  • Mitogens
  • RNA, Messenger
  • Cyclin-Dependent Kinases