Abstract
Since T lymphocytes are active producers of regulatory cytokines, long-term T cell cultures (LTTC) specific for a major mannoproteic antigen (MP) of Candida albicans from peripheral blood mononuclear cells (PBMC) of healthy donors were generated and their cytokine profile studied. LTTC consisted of an expanded CD3CD4 T-helper (Th) populations, with a high proportion of CD45R0 T memory cells. Stimulation of LTTC by MP induced a Th-1 type cytokine profile, as indicated by the presence of IFN-gamma and the absence of IL-5 (both as mRNA and protein) in cell cultures and supernatants. These results suggest a predominant Th1 response elicited by C. albicans mannoprotein antigen in human PBMC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Fungal / immunology*
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Antigens, Fungal / isolation & purification
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Candida albicans / chemistry
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Candida albicans / immunology*
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Cells, Cultured
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Gene Expression Regulation
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Humans
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Immunologic Memory
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Interferon-gamma / biosynthesis
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Interferon-gamma / genetics
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Interleukin-2 / pharmacology
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Leukocyte Common Antigens / analysis
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Lymphocyte Activation
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Membrane Glycoproteins / immunology*
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Membrane Glycoproteins / isolation & purification
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Methylprednisolone / pharmacology
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Th1 Cells / drug effects*
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Th1 Cells / immunology
Substances
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Antigens, Fungal
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Interleukin-2
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Membrane Glycoproteins
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RNA, Messenger
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mannoproteins
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Interferon-gamma
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Leukocyte Common Antigens
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Methylprednisolone