Abstract
Monoclonal antibodies (MAbs) against quail Pax-1 were generated using a fusion protein of the C-terminal part of Pax-1 and glutathione S-transferase. The MAbs generated could detect quail Pax-1 protein by Western blotting and immunoprecipitate it from whole cell extracts of quail embryos, indicating their usefulness for biochemical analyses of Pax-1 protein function in development or oncogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / chemistry
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Blotting, Western
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / immunology*
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DNA-Binding Proteins / isolation & purification
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Immunoglobulin Isotypes / chemistry
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Paired Box Transcription Factors
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Precipitin Tests
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Quail / genetics
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Quail / immunology*
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Transcription Factors / genetics
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Transcription Factors / immunology*
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Transcription Factors / isolation & purification
Substances
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Antibodies, Monoclonal
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DNA-Binding Proteins
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Immunoglobulin Isotypes
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Paired Box Transcription Factors
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Transcription Factors
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PAX1 transcription factor