Tissue homogenization as a key step in extracting RNA from human and rat pancreatic tissue

Biotechniques. 1997 Mar;22(3):408-10, 412. doi: 10.2144/97223bm07.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Extracts / chemistry
  • Electrophoresis, Agar Gel
  • Guanidine
  • Guanidines
  • Humans
  • Male
  • Organ Preservation Solutions
  • Organotin Compounds / pharmacology
  • Pancreas / chemistry*
  • Pancreatitis / chemically induced
  • Protein Denaturation
  • RNA / isolation & purification*
  • RNA, Ribosomal, 18S / isolation & purification
  • RNA, Ribosomal, 28S / isolation & purification
  • Rats
  • Rats, Inbred Lew
  • Specimen Handling / methods
  • Spectrophotometry
  • Tissue Preservation

Substances

  • Cell Extracts
  • Guanidines
  • Organ Preservation Solutions
  • Organotin Compounds
  • RNA, Ribosomal, 18S
  • RNA, Ribosomal, 28S
  • di-n-butyltin
  • RNA
  • dibutyldichlorotin
  • Guanidine