Abstract
DNA-damaging agents induce apoptosis primarily by a p53-dependent pathway. LTR6 cells containing a temperature-sensitive p53 were used to dissect further the mechanisms of p53-induced apoptosis. Apoptosis was accompanied by the processing and activation of CPP32 and Mch3 alpha, together with the cleavage of poly(ADP-ribose) polymerase and lamin B1. These results demonstrate a critical role for the activation of interleukin-1 beta-converting enzyme-like proteases in p53-induced apoptosis.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Apoptosis* / drug effects
-
Caspase 3
-
Caspases*
-
Coumarins / metabolism
-
Cysteine Endopeptidases / metabolism*
-
Enzyme Activation / drug effects
-
Fluorescent Dyes
-
Leukemia, Myeloid
-
Mice
-
Oligopeptides / metabolism
-
Substrate Specificity
-
Tumor Cells, Cultured
-
Tumor Suppressor Protein p53 / pharmacology*
Substances
-
Coumarins
-
Fluorescent Dyes
-
Oligopeptides
-
Tumor Suppressor Protein p53
-
acetyl-aspartyl-glutamyl-valyl-aspartyl-amino-4-methylcoumarin
-
Casp3 protein, mouse
-
Caspase 3
-
Caspases
-
Cysteine Endopeptidases