Abstract
In mating experiments using a clinical strain that constitutively expresses vanB-encoded glycopeptide resistance, resistance transfer was detectable at a frequency of <10(-7) transconjugants/donor. Vancomycin MICs for transconjugants were 2- to 10-fold lower than those for the donor; both inducibly and constitutively resistant transconjugants were obtained. These findings demonstrate that the transfer of vanB among enterococci can be associated with substantial alterations in the level and control of glycopeptide resistance expression.
MeSH terms
-
Anti-Bacterial Agents / pharmacology*
-
Chromosomes, Bacterial / chemistry
-
Conjugation, Genetic / genetics*
-
DNA, Bacterial / genetics
-
DNA, Bacterial / metabolism
-
Drug Resistance, Microbial
-
Electrophoresis, Polyacrylamide Gel
-
Enterococcus faecium / drug effects*
-
Enterococcus faecium / genetics*
-
Gene Expression Regulation, Bacterial / genetics
-
Gene Expression Regulation, Bacterial / physiology
-
Genes, Bacterial / genetics*
-
Glycopeptides*
-
Polymerase Chain Reaction
Substances
-
Anti-Bacterial Agents
-
DNA, Bacterial
-
Glycopeptides