Complete sequence of SpHox8 and its linkage in the single Hox gene cluster of Strongylocentrotus purpuratus

J Mol Evol. 1997 Apr;44(4):371-7. doi: 10.1007/pl00006156.

Abstract

SpHox8 is the paralog group 8 Hox gene of Strongylocentrotus purpuratus. This identification follows from an analysis of the sequence of the complete open reading frame of a late-gastrula-stage cDNA clone; from direct linkage to adjacent Hox genes in a fosmid contig; and from blot hybridizations carried out on pulse field gel electrophoretic separations. SpHox8 is a single-copy gene, and there is only one Hox gene cluster per genome in S. purpuratus.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA Primers
  • DNA, Complementary
  • Genes, Homeobox*
  • Genetic Linkage
  • Homeodomain Proteins / chemistry
  • Homeodomain Proteins / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Recombinant Proteins / chemistry
  • Sea Urchins / genetics*
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • DNA, Complementary
  • Homeodomain Proteins
  • Recombinant Proteins
  • SpHox8 protein, Strongylocentrotus purpuratus

Associated data

  • GENBANK/D85419

Grants and funding