Abstract
Activation of CD4(+) T lymphocytes from human immunodeficiency virus-type 1 (HIV-1)-infected donors with immobilized antibodies to CD3 and CD28 induces a virus-resistant state. This effect is specific for macrophage-tropic HIV-1. Transcripts encoding CXCR4/Fusin, the fusion cofactor used by T cell line-tropic isolates, were abundant in CD3/CD28-stimulated cells, but transcripts encoding CCR5, the fusion cofactor used by macrophage-tropic viruses, were not detectable. Thus, CD3/CD28 costimulation induces an HIV-1-resistant phenotype similar to that seen in some highly exposed and HIV-uninfected individuals.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antibodies, Monoclonal / immunology
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CD28 Antigens / immunology*
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CD3 Complex / immunology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / virology*
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Cells, Cultured
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Gene Expression Regulation
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HIV-1 / physiology*
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Humans
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Interleukin-2 / immunology
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Lymphocyte Activation*
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Membrane Fusion
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Membrane Proteins / genetics*
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Muromonab-CD3 / immunology
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Phytohemagglutinins / pharmacology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, CCR5
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Receptors, CXCR4
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Receptors, Cytokine / genetics
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Receptors, HIV / genetics*
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Up-Regulation
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Virus Replication
Substances
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Antibodies, Monoclonal
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CD28 Antigens
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CD3 Complex
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Interleukin-2
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Membrane Proteins
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Muromonab-CD3
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Phytohemagglutinins
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RNA, Messenger
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Receptors, CCR5
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Receptors, CXCR4
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Receptors, Cytokine
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Receptors, HIV