Granulocyte macrophage colony stimulating factor pretreatment induces an increase of rat Kupffer cells with enhanced cytotoxicity in vitro and prevention of tumor outgrowth in vivo

Anticancer Drugs. 1997 Mar;8(3):269-75. doi: 10.1097/00001813-199703000-00008.

Abstract

Male Wag rats were pretreated for 7 days with 1000 U/ml recombinant murine granulocyte macrophage colony stimulating factor (rmGM-CSF). Rat Kupffer cells (KC) were isolated by a enzymatic method. We injected decreasing numbers of CC531 tumor cells in the portal system. Mean KC yield increased from 1.5 +/- 0.2 to 2.2 +/- 0.2 (p < 0.05). Mean percentage of KC-mediated cytotoxicity against CC531 increased from 20.0 +/- 0.5 to 42 +/- 1.0 after rmGM-CSF (p < 0.05). At 1 x 10(5) CC531 tumor cells we demonstrated prevention of formation of small foci of CC52+ tumor cells. We demonstrate increased isolated KC with enhanced cytotoxic capacity after rmGM-CSF. rmGM-CSF induced prevention of minimal residual disease in the rat liver.

MeSH terms

  • Animals
  • Carcinoma / drug therapy
  • Cell Death / drug effects
  • Drug Screening Assays, Antitumor
  • Granulocyte Colony-Stimulating Factor / pharmacology*
  • Granulocyte Colony-Stimulating Factor / therapeutic use
  • Immunohistochemistry
  • Kupffer Cells / drug effects*
  • Liver Neoplasms, Experimental / prevention & control
  • Liver Neoplasms, Experimental / secondary
  • Neoplasm Transplantation
  • Rats
  • Rats, Inbred Strains
  • Recombinant Proteins
  • Tetrazolium Salts
  • Thiazoles
  • Thymidine / metabolism
  • Tumor Cells, Cultured

Substances

  • Recombinant Proteins
  • Tetrazolium Salts
  • Thiazoles
  • Granulocyte Colony-Stimulating Factor
  • thiazolyl blue
  • Thymidine