Alternate splicing of mouse fusin/CXC chemokine receptor-4: stromal cell-derived factor-1alpha is a ligand for both CXC chemokine receptor-4 isoforms

J Immunol. 1997 Apr 15;158(8):3561-4.

Abstract

Inspection of the intron splice junction of the mouse chemokine receptor fusin/CXC chemokine receptor R-4 (CXCR-4) revealed a potential in-frame alternative splice site in the region encoding the N-terminal ectodomain of the receptor. Both predicted splice products were detected by reverse transcriptase-PCR. Cell lines of T lymphocyte, B lymphocyte, and macrophage lineage, plus populations of elicited peritoneal exudate cells, thymocytes, and astrocytes coexpressed mRNA for both isoforms. The full length cDNA of the shorter alternate splice product, termed CXCR-4B, was cloned from peritoneal exudate cell RNA. Chinese hamster ovary cells transfected with either isoform, CXCR-4A or CXCR-4B, responded to stromal cell-derived factor-1alpha with a rise in intracellular calcium. Both alternate splice products are therefore functional stromal cell-derived factor-1 alpha receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing*
  • Animals
  • Chemokine CXCL12
  • Chemokines / metabolism*
  • Chemokines, CXC*
  • Cricetinae
  • Gene Expression Regulation
  • Ligands
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mice
  • Molecular Sequence Data
  • Receptors, CXCR4
  • Receptors, HIV / genetics*
  • Receptors, HIV / metabolism

Substances

  • Chemokine CXCL12
  • Chemokines
  • Chemokines, CXC
  • Cxcl12 protein, mouse
  • Ligands
  • Membrane Proteins
  • Receptors, CXCR4
  • Receptors, HIV

Associated data

  • GENBANK/U65580