Abstract
In this report, we demonstrate stimulation of T cell receptor (TCR) transgenic CD8 T cells by isolated major histocompatibility complex (MHC) class I H-2Ld complexes and antigenic peptide. This is the first demonstration of CD8 T cells activated by MHC and antigenic peptide in the absence of antigen priming. Furthermore, isolated MHC and a potent peptide antigen can stimulate phenotypically naive CD44- T cells to become CTL effectors and to produce interleukin-2 in nanogram per milliliter amounts. These results demonstrate that particular TCR antigen pairs may overcome the need for specialized antigen-presenting cells and have implications for mechanisms of autoimmunity and tolerance induction.
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology
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CD8-Positive T-Lymphocytes / classification
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CD8-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / metabolism
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Dose-Response Relationship, Immunologic
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Female
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H-2 Antigens / immunology*
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H-2 Antigens / isolation & purification
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H-2 Antigens / physiology
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Histocompatibility Antigen H-2D
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Hyaluronan Receptors
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Immunophenotyping
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Interleukin-2 / biosynthesis
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Isoantigens / immunology
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Ketoglutarate Dehydrogenase Complex / immunology*
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Lymphocyte Activation*
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Oligopeptides / immunology*
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Oligopeptides / isolation & purification
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Oligopeptides / pharmacology
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T-Lymphocytes, Cytotoxic / immunology
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Titrimetry
Substances
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H-2 Antigens
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H-2Kb protein, mouse
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Histocompatibility Antigen H-2D
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Hyaluronan Receptors
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Interleukin-2
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Isoantigens
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Oligopeptides
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p2Ca peptide
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Ketoglutarate Dehydrogenase Complex