Abstract
A new method for the purification of bovine angiogenin is proposed which is based on the use of CM-cellulose, CM-Toyopearl, and Butyl-Toyopearl. Electrophoretically homogeneous protein was obtained with a 49% yield and 12,000-fold purification.
MeSH terms
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Angiogenesis Inducing Agents / isolation & purification*
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Animals
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Chromatography, DEAE-Cellulose
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Milk / chemistry*
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Proteins / isolation & purification*
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Ribonuclease, Pancreatic*
Substances
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Angiogenesis Inducing Agents
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Proteins
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angiogenin
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Ribonuclease, Pancreatic