Abstract
STAT (signal transducers and activators of transcription) proteins undergo cytokine-dependent phosphorylation on serine and tyrosine. STAT3, a transcription factor for acute phase response genes, was found to act as an adapter molecule in signal transduction from the type I interferon receptor. STAT3 bound to a conserved sequence in the cytoplasmic tail of the IFNAR1 chain of the receptor and underwent interferon-dependent tyrosine phosphorylation. The p85 regulatory subunit of phosphatidylinositol 3-kinase, which activates a series of serine kinases, bound to phosphorylated STAT3 and subsequently underwent tyrosine phosphorylation. Thus, STAT3 acts as an adapter to couple another signaling pathway to the interferon receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute-Phase Proteins / genetics*
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Amino Acid Sequence
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Androstadienes / pharmacology
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Animals
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Binding Sites
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COS Cells
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Cell Line
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Cloning, Molecular
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Conserved Sequence
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Enzyme Inhibitors / pharmacology
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Membrane Proteins
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Molecular Sequence Data
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Phosphatidylinositol 3-Kinases
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Phosphorylation
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Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Point Mutation
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Protein Binding
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Receptor, Interferon alpha-beta
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Receptors, Interferon / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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STAT3 Transcription Factor
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Signal Transduction
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Tyrosine / metabolism
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Wortmannin
Substances
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Acute-Phase Proteins
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Androstadienes
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DNA-Binding Proteins
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Enzyme Inhibitors
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Membrane Proteins
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Receptors, Interferon
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Recombinant Fusion Proteins
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STAT3 Transcription Factor
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Trans-Activators
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Receptor, Interferon alpha-beta
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Tyrosine
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Phosphatidylinositol 3-Kinases
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Phosphotransferases (Alcohol Group Acceptor)
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Wortmannin