Enhancement of interleukin-1 alpha mediated autocrine growth of cultured human keratinocytes by sho-saiko-to

Jpn J Pharmacol. 1997 Apr;73(4):333-6. doi: 10.1254/jjp.73.333.

Abstract

We investigated the effects of Sho-saiko-to, the most commonly used herbal medicine in Japan, on the production of interleukin (IL)-1 alpha by cultured human epidermal keratinocytes. IL-1 alpha production was significantly promoted by treatment with 100 or 500 micrograms/ml Sho-saiko-to for 24 or 48 hr. Expression of IL-1 alpha receptors was the most markedly upregulated after treatment with 500 micrograms/ml Sho-saiko-to for 24 hr and with 100 or 500 micrograms/ml for 48 hr; these cells showed the characteristics of multilayered differentiated keratinocytes. The presence of an anti-IL-1 alpha antibody during the treatment with 500 micrograms/ml of Sho-saiko-to for 24 or 48 hr or with 100 micrograms/ml for 48 hr significantly down-regulated the synthesis by the keratinocytes and induced damages in them. Keratinocytes treated with Sho-saiko-to might produce IL-1 alpha and express IL-1 alpha receptors. IL-1 alpha may regulate the proliferation and differentiation of keratinocytes after Sho-saiko-to treatment. These findings suggest that Sho-saiko-to enhances the autocrine growth mediated by IL-1 alpha.

MeSH terms

  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Antibodies / toxicity
  • Antineoplastic Agents / pharmacology*
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cells, Cultured
  • DNA / biosynthesis
  • Down-Regulation
  • Drugs, Chinese Herbal / pharmacology*
  • Epidermal Cells
  • Epidermis / drug effects
  • Humans
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / immunology
  • Interleukin-1 / physiology
  • Keratinocytes / cytology
  • Keratinocytes / drug effects*
  • Microscopy, Phase-Contrast
  • Receptors, Interleukin-1 / biosynthesis*
  • Receptors, Interleukin-1 / genetics
  • Receptors, Interleukin-1 / immunology
  • Up-Regulation

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Antibodies
  • Antineoplastic Agents
  • Drugs, Chinese Herbal
  • Interleukin-1
  • Receptors, Interleukin-1
  • saiko-keishi-to
  • DNA