The protein encoded by the MFT1 gene is a targeting factor for mitochondrial precursor proteins, and not a core ribosomal protein

T Beilharz; T Beddoe; K Landl; P Cartwright; T Lithgow

doi:10.1016/s0014-5793(97)00349-9

The protein encoded by the MFT1 gene is a targeting factor for mitochondrial precursor proteins, and not a core ribosomal protein

FEBS Lett. 1997 Apr 28;407(2):220-4. doi: 10.1016/s0014-5793(97)00349-9.

Authors

T Beilharz¹, T Beddoe, K Landl, P Cartwright, T Lithgow

Affiliation

¹ School of Biochemistry, La Trobe University, Bundoora, Australia.

PMID: 9166903
DOI: 10.1016/s0014-5793(97)00349-9

Abstract

Yeast cells harboring mft1 mutations are compromised in mitochondrial protein targeting, and Mft1p has previously been identified as a ribosomal protein. However, two genes, PLC2 and YML062C, are present in the MFT1 locus, and we show that mft1 mutant cells are compromised in the function of the cytosolic protein encoded by YML062C. The ribosomal protein (YS3a) is actually encoded by the tightly linked PLC2 gene, and does not play a role in targeting proteins to the mitochondria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Cell Compartmentation*
DNA-Binding Proteins*
Fungal Proteins / genetics*
Genes, Fungal*
Genetic Linkage
Mitochondria / metabolism*
Molecular Sequence Data
Mutagenesis, Insertional
Protein Binding
Protein Biosynthesis
Protein Precursors / metabolism*
Ribosomal Proteins / genetics
Saccharomyces cerevisiae Proteins*
Sequence Homology, Amino Acid
Yeasts / genetics*

Substances

DNA-Binding Proteins
Fungal Proteins
Protein Precursors
Ribosomal Proteins
Saccharomyces cerevisiae Proteins
MFT1 protein, S cerevisiae