Abstract
During the final step of the malignant progression to glioblastoma multiforme (GBM), the most frequent and malignant of primary brain tumors, more than 90% of the cases exhibit loss of genetic material on chromosome 10. We previously identified a 4-cM deletion interval in the 10q24-qter region that is common to all the GBM we have examined. A contig of 20 YACs spanning the 5 Mb of chromosomal DNA in the region has been assembled. Overlaps between YACs have been verified by STS content, fingerprinting analysis, and/or Alu-Alu PCR. The contig contains 17 known microsatellite markers, 15 new STSs derived from the insert ends of YACs, 9 ESTs, and 11 others STSs, for a total of 52 STSs (average marker density 1/100 kb). The physical map of this region will facilitate the search for a candidate tumor-suppressor gene(s) that is inactivated during the information of GBM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Brain Neoplasms / genetics*
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Chromosome Mapping
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Chromosomes, Artificial, Yeast / genetics*
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Chromosomes, Human, Pair 10 / genetics*
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DNA Primers / genetics
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DNA, Complementary / genetics
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DNA, Neoplasm / genetics
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Genes, Tumor Suppressor*
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Genetic Markers
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Glioblastoma / genetics*
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Humans
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Molecular Sequence Data
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Sequence Tagged Sites
Substances
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DNA Primers
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DNA, Complementary
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DNA, Neoplasm
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Genetic Markers
Associated data
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GENBANK/G04397
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GENBANK/G04646
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GENBANK/G08792
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GENBANK/G08796
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GENBANK/G08809
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