Abstract
Murine sarcoma cell line (L-1) treated with promoting phorbol ester (TPA) showed decreased content and activity of protein kinase C (PKC) as measured by Western blotting and histone phosphorylation methods. The PKC depleted line (L-1R) produced bigger, tumors after s.c. transplantation into syngeneic mice and more spontaneous and artificial metastases developing after i.v. injection of tumor cells. The in vitro studies revealed decreased: adhesiveness, migratory and invasiveness properties of PKC depleted cells. Negative correlation between in vitro and in vivo studies were found.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Carcinogens / pharmacology*
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Cell Adhesion / drug effects
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Cell Movement / drug effects
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Female
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Lung Neoplasms / secondary
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Mice
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Mice, Inbred BALB C
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Neoplasm Transplantation
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Phosphorylation
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Protein Kinase C / drug effects
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Protein Kinase C / metabolism
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Sarcoma, Experimental / drug therapy*
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Sarcoma, Experimental / enzymology
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Sarcoma, Experimental / pathology*
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Tetradecanoylphorbol Acetate / pharmacology*
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Tumor Cells, Cultured / drug effects
Substances
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Carcinogens
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Protein Kinase C
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Tetradecanoylphorbol Acetate