Conversion of in vitro cultured human monocytes into effective presenters of an HER2/neu-encoded CTL peptide epitope

Scand J Immunol. 1997 Jun;45(6):678-82. doi: 10.1046/j.1365-3083.1997.d01-450.x.

Abstract

Tumour-derived peptides have been surveyed, in a variety of systems, for their ability to elicit cytokine release from class I restricted T cells. Analogous studies on ovarian carcinoma have employed the antigen-processing defective T2 cell line, Purified dendritic cells (DC) have been reported to act as highly effective APC. A facile method was developed whereby DC-like cells were generated from monocyte precursors. Herein, evidence is presented suggesting DC-like cells are superior to T2 with respect to their ability to present a defined CTL epitope associated with ovarian carcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen-Presenting Cells / metabolism*
  • Carcinoma
  • Cell Culture Techniques / methods
  • Culture Media
  • Cytokines / pharmacology
  • Dendritic Cells / metabolism
  • Epitopes, T-Lymphocyte / immunology*
  • Female
  • Gelatin
  • Genes, erbB-2 / immunology*
  • Humans
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Ovarian Neoplasms
  • Peptides / immunology*
  • Receptor, ErbB-2 / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • Tumor Cells, Cultured

Substances

  • Culture Media
  • Cytokines
  • Epitopes, T-Lymphocyte
  • Peptides
  • Gelatin
  • Receptor, ErbB-2