Abstract
Brefeldin A (BFA) decreased the expression of influenza A virus haemagglutinin (HA) and M2 protein on the plasma membrane of virus-infected MDCK cells. It caused a retention of M1 protein in the cell nucleus and a decrease of its expression on the plasma membrane. On the other hand, an increased labelling of the cytoplasmic domain of M2 protein on the plasma membrane in BFA-treated cells was observed in contrast to the labelling in BFA-untreated cells. The effects of BFA on the microtubules and cellular motors are discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Surface / metabolism
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Biological Transport / drug effects
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Brefeldin A
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Cell Compartmentation / drug effects
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Cell Line
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Cyclopentanes / pharmacology*
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Dogs
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Hemagglutinins, Viral / metabolism*
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Immunohistochemistry
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Ion Channels / metabolism
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Membrane Proteins / metabolism
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Viral Matrix Proteins / metabolism*
Substances
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Antigens, Surface
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Cyclopentanes
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Hemagglutinins, Viral
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Ion Channels
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M-protein, influenza virus
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M1 protein, Influenza A virus
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M2 protein, Influenza A virus
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Membrane Proteins
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Viral Matrix Proteins
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Brefeldin A