Identification by polymerase chain reaction and hybridization with sequence-specific oligoprobes and complete exon-2 cDNA sequencing of a new DRB1 (DRB1*1131) allele

Tissue Antigens. 1997 Jul;50(1):96-8. doi: 10.1111/j.1399-0039.1997.tb02845.x.

Abstract

HLA-DRB1 is the most polymorphic gene described so far, and their encoded molecules disclose a major role in allogeneic responses. We describe in this report a new DRB1 allele in a Spanish Caucasian bone marrow donor, initially defined by PCR-SSO as a DRB1*11-like allele. Complete exon 2 cDNA-sequencing reveled that this allele was identical to DRB1*1119 except for a single substitution at position 178, which generates an amino acid change (Tyr-His) at position 60. This residue is shared by several DRB1*14 subtypes and DRB1*0808. The new allele was officially named DRB1*1131.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Amino Acid Sequence
  • Base Sequence
  • Cell Line, Transformed
  • DNA, Complementary
  • Exons*
  • HLA-DR Antigens / genetics*
  • HLA-DRB1 Chains
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • Polymerase Chain Reaction

Substances

  • DNA, Complementary
  • HLA-DR Antigens
  • HLA-DRB1 Chains
  • Oligonucleotide Probes

Associated data

  • GENBANK/U72064