Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

N C Heng; H F Jenkinson; G W Tannock

doi:10.1128/aem.63.8.3336-3340.1997

Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

Appl Environ Microbiol. 1997 Aug;63(8):3336-40. doi: 10.1128/aem.63.8.3336-3340.1997.

Authors

N C Heng¹, H F Jenkinson, G W Tannock

Affiliation

¹ Department of Microbiology, University of Otago, Dunedin, New Zealand.

Abstract

Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus / genetics*
Cloning, Molecular
DNA Probes / genetics
Endo-1,3(4)-beta-Glucanase*
Gene Expression
Glycoside Hydrolases / genetics*
Glycoside Hydrolases / metabolism*
Lactobacillus / genetics*
Lactobacillus / metabolism*
Plasmids
Promoter Regions, Genetic / genetics
Recombination, Genetic
Restriction Mapping
beta-Galactosidase / metabolism

Substances

DNA Probes
Glycoside Hydrolases
beta-Galactosidase
Endo-1,3(4)-beta-Glucanase