Identification of surface tegumental antigens of normal and irradiated schistosomula

J Egypt Soc Parasitol. 1997 Aug;27(2):479-91.

Abstract

Sodium dodecyl sulphate- poly acrylamide gel electronphoresis (SDS-PAGE) fractionation of tegumental surface antigens (STEG-Ags.) of 7-day cultured normal and irradiated schistosomula showed no obvious qualitative differences. The observed polypeptide bands of both normal irradiated STEG-Ags. were almost identical and have similar corresponding molecular weights. The immunoblotting assay, using different types of mouse sera, revealed similarity between the bands of both normal and irradiated STEG-Ags. recognized by each type of mouse serum. No qualitative rather than quantitative differences have been observed. The quantitative differences were reflected in intensively staining of some bands from normal STEG-Ag. rather than their corresponding bands of the same molecular weights from irradiated STEG-Ag.

MeSH terms

  • Animals
  • Antibodies, Helminth
  • Antigens, Helminth / analysis*
  • Antigens, Helminth / radiation effects
  • Antigens, Surface / analysis*
  • Antigens, Surface / radiation effects
  • Electrophoresis, Polyacrylamide Gel
  • Immunoblotting
  • Mice
  • Mice, Inbred BALB C
  • Schistosoma mansoni / chemistry*
  • Schistosoma mansoni / radiation effects*

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Antigens, Surface