Abstract
32D cells are interleukin-3 (IL-3) dependent murine hemopoietic cells, that undergo apoptosis after IL-3 withdrawal. An overexpressed insulin-like growth factor I receptor (IGF-IR) protects these cells from apoptosis induced by IL-3 withdrawal. When 32D cells are stably transfected with plasmids expressing either IRS-1 (a major substrate of the IGF-IR) or the Simian virus 40 large T antigen, singly, they still undergo apoptosis after IL-3 withdrawal, although IRS-1 offers partial protection. The cells, however, are fully protected when they are stably transfected with both IRS-1 and SV40 T antigen. Protection from apoptosis in these cells is characterized by the stabilization of the Stat1 and Stat5 protein levels, whose synthesis is inhibited when IL-3 is withdrawn.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Polyomavirus Transforming / pharmacology
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Antigens, Polyomavirus Transforming / physiology*
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Apoptosis*
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Cell Division
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Cell Line / drug effects
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DNA-Binding Proteins / biosynthesis
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Insulin Receptor Substrate Proteins
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Interleukin-3 / pharmacology*
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Methionine / analysis
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Mice
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Milk Proteins*
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Phosphoproteins / pharmacology
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Phosphoproteins / physiology*
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Plasmids
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STAT1 Transcription Factor
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STAT5 Transcription Factor
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Trans-Activators / biosynthesis
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Transfection
Substances
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Antigens, Polyomavirus Transforming
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DNA-Binding Proteins
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Insulin Receptor Substrate Proteins
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Interleukin-3
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Irs1 protein, mouse
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Milk Proteins
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Phosphoproteins
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STAT1 Transcription Factor
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STAT5 Transcription Factor
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Stat1 protein, mouse
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Trans-Activators
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Methionine