Retroviral particles produced from a stable human-derived packaging cell line transduce target cells with very high efficiencies

Hum Gene Ther. 1997 Aug 10;8(12):1459-67. doi: 10.1089/hum.1997.8.12-1459.

Abstract

The goal of this work was to determine whether a stable 293 amphotropic packaging line, which we have designated 293-SPA, is useful for the production of high-titer stable virus by comparison to the murine psiCRIP line. Here, we report our unexpected findings that particles derived from the 293-SPA line transduce target cells (both NIH-3T3 cells and primary melanoma cells) with greatly enhanced efficiencies (at least 10-fold) compared to particles derived from the psiCRIP packaging line. We show that the presence of a transferable inhibitor in the psiCRIP line at least partially accounts for this dramatic difference in transduction efficiency. This work has important implications for improving the efficiency of retrovirus-mediated gene transfer in general as well as in the design of new packaging cell lines.

Publication types

  • Comparative Study

MeSH terms

  • 3T3 Cells / virology
  • Animals
  • Cell Line
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
  • Humans
  • Kidney / cytology*
  • Kidney / embryology
  • Kidney / virology*
  • Melanoma / genetics
  • Melanoma / metabolism
  • Melanoma / virology
  • Mice
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Retroviridae / genetics*
  • Transduction, Genetic*
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Recombinant Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • beta-Galactosidase