Genome analysis in Neurospora crassa; cloning of four loci arginine-1 (arg-1), methionine-6 (met-6), unknown-7 (un-7), and ribosome production-1 (rip-1) and associated chromosome walking

Fungal Genet Biol. 1997 Jun;21(3):323-8. doi: 10.1006/fgbi.1997.0974.

Abstract

We have cloned four Neurospora crassa genes by complementation analysis. Cloned genes include the arginine-1 (arg-1), methionine-6 (met-6), unknown-7 (un-7), and ribosome production-1 (rip-1) loci. Chromosome walks were initiated in ordered cosmid libraries from the cloned loci. A total of about 700 kb of the Neurospora genome is covered in these walks.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arginine / genetics*
  • Chromosome Mapping
  • Chromosome Walking
  • Chromosomes, Fungal*
  • Cloning, Molecular / methods
  • Cosmids
  • Gene Library
  • Genetic Markers
  • Genome, Fungal*
  • Methionine / genetics*
  • Neurospora crassa / genetics*
  • Polymorphism, Restriction Fragment Length
  • Ribosomes

Substances

  • Genetic Markers
  • Arginine
  • Methionine