Abstract
Objective:
To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes.
Methods:
Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis.
Results:
IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA.
Conclusion:
IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Cartilage, Articular / drug effects*
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Cartilage, Articular / enzymology*
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Cell Division / drug effects
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Cells, Cultured
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Dose-Response Relationship, Drug
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Glycoproteins / genetics
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Glycoproteins / metabolism
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Growth Substances / pharmacology
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Humans
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Interleukin-1 / antagonists & inhibitors
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Interleukin-1 / pharmacology
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Interleukin-4 / pharmacology*
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Matrix Metalloproteinase 3 / genetics
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Matrix Metalloproteinase 3 / metabolism*
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Metalloendopeptidases / metabolism
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Protease Inhibitors / metabolism
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RNA, Messenger / biosynthesis
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Tissue Inhibitor of Metalloproteinases
Substances
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Glycoproteins
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Growth Substances
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Interleukin-1
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Protease Inhibitors
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RNA, Messenger
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Tissue Inhibitor of Metalloproteinases
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Interleukin-4
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Metalloendopeptidases
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caseinase
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Matrix Metalloproteinase 3