Abstract
A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the level of the mucosa itself.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cervix Mucus / virology*
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Female
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HIV / isolation & purification*
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HIV / physiology
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HIV Infections / physiopathology*
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HIV Infections / virology
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Humans
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Polymerase Chain Reaction
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RNA, Viral / analysis
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RNA-Directed DNA Polymerase
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Therapeutic Irrigation
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Virus Shedding* / genetics
Substances
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RNA, Viral
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RNA-Directed DNA Polymerase