Does umbilical cord blood polymerase chain reaction positivity indicate in utero (pre-labor) HIV infection?

AIDS. 1997 Sep;11(11):1375-82. doi: 10.1097/00002030-199711000-00012.

Abstract

Objective: To compare risk factors for infants whose cord blood was positive for HIV DNA with those who were cord blood-negative but found to be HIV DNA-positive in early infancy.

Methods: In 1994, infants born to HIV-infected women were enrolled in a study in Blantyre, Malawi. Birth weight and transmission risk factors from cord blood-positive infants were compared with cord blood-negative/HIV-positive infants on their first postnatal visit (4-7 weeks of age). Testing for HIV DNA on cord and peripheral blood was performed by polymerase chain reaction.

Results: Of 249 HIV-infected infants (overall transmission rate, 26%), 83 (33%) were cord blood-positive and 166 were initially cord blood-negative. The mean birth weight was 2.1% (59 g) lighter in cord blood-positive infants than initially cord blood-negative infants; initially cord blood-negative infants were 2.8% (80 g) lighter than uninfected infants born to HIV-infected women. There were no significant differences in the risk factors for infection between HIV-infected cord blood-positive and -negative infants; when transmission was increased, both HIV-infected cord blood-positive and -negative infants contributed to the increase in a similar proportion.

Interpretation: It was concluded that umbilical cord blood positivity for HIV DNA did not identity a subset of in utero HIV-infected infants and suggested that HIV-infected cord blood-positive and -negative infants have similar timing and routes of HIV infection.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Birth Weight
  • DNA, Viral / isolation & purification
  • Female
  • Fetal Blood / immunology*
  • Fetal Blood / virology*
  • HIV / immunology
  • HIV / isolation & purification*
  • HIV Antibodies / immunology
  • HIV Infections / diagnosis
  • HIV Infections / epidemiology*
  • HIV Infections / transmission*
  • HIV Seronegativity
  • HIV Seropositivity
  • Humans
  • Infant
  • Infant, Newborn
  • Infectious Disease Transmission, Vertical
  • Male
  • Polymerase Chain Reaction
  • Pregnancy
  • Pregnancy Complications, Infectious / virology
  • Risk Factors

Substances

  • DNA, Viral
  • HIV Antibodies