A novel MLL-AF10 fusion mRNA variant in a patient with acute myeloid leukemia detected by a new asymmetric reverse transcription PCR method

Leukemia. 1997 Sep;11(9):1588-93. doi: 10.1038/sj.leu.2400758.

Abstract

A novel variant of the chimerical MLL-AF10 mRNA transcript was detected in a pediatric patient with acute myeloid leukemia (AML) by a new asymmetric reverse-transcription polymerase chain reaction (ART-PCR) method. Sequence analysis of the fusion region on the amplified cDNA fragment showed an in-frame joining of exon e5 of the MLL gene and position 1931 of the cDNA sequence of the AF10 gene, giving rise to a new MLL-AF10 transcript. The presence of the new chimerical mRNA product in a sample from the patient was confirmed by classical RT-PCR.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Amino Acid Sequence
  • Child
  • Chromosomes, Human, Pair 10
  • Chromosomes, Human, Pair 11
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics*
  • Histone-Lysine N-Methyltransferase
  • Humans
  • Leukemia, Myeloid / genetics*
  • Molecular Sequence Data
  • Myeloid-Lymphoid Leukemia Protein
  • Polymerase Chain Reaction / methods*
  • Proto-Oncogenes*
  • RNA, Neoplasm / genetics
  • Recombinant Fusion Proteins
  • Restriction Mapping
  • Transcription Factors / genetics*
  • Translocation, Genetic

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • KMT2A protein, human
  • MLLT10 protein, human
  • RNA, Neoplasm
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Myeloid-Lymphoid Leukemia Protein
  • Histone-Lysine N-Methyltransferase

Associated data

  • GENBANK/U13948