Polymorphism at codons 114, 116, 145, and 163 muddle the typing of HLA-B*1304

Hum Immunol. 1997 Jun;55(1):66-73. doi: 10.1016/s0198-8859(97)00055-4.

Abstract

Genetic exchanges often muddle the typing of HLA class I molecules, this is also the case for HLA-B*1304. Serologic and molecular DNA class I typing report a B15/B55 type for cell 847, whereas DNA sequencing finds B*5501/B*1304. HLA-B*1304 differs by no more than four amino acids from other HLA-B13 molecules, a comparative analysis of the B13 and B15 families was therefore performed to determine why serologic and molecular DNA approaches report a B15 type for B*1304. Comparisons demonstrate that limited differences individuate the B15 and B13 molecules such that the genetic recombination of codons 145 and 163 in the class I heavy chain's alpha 2 alpha helix prompt B*1304 to exhibit a B15X21 pattern of serologic cross-reactivity. Molecular DNA class I typing approaches are also swayed by genetic recombinations to type B*1304 as a B15 molecule: B15-like nucleotide sequences encoding residues 114, 116, and 145, lead B*1304 to exhibit a B15 PCR amplification pattern. Thus, genetic exchanges encoding key amino acids in the class I heavy chain lead molecular and serologic typing approaches to categorize HLA-B*1304 as a member of the B15 family.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Codon*
  • Cross Reactions
  • Genotype
  • HLA-B Antigens / genetics*
  • HLA-B Antigens / immunology
  • HLA-B13 Antigen
  • HLA-B15 Antigen
  • Histocompatibility Testing
  • Humans
  • Molecular Sequence Data
  • Polymorphism, Genetic*

Substances

  • Codon
  • HLA-B Antigens
  • HLA-B13 Antigen
  • HLA-B15 Antigen
  • HLA-B21 antigen

Associated data

  • GENBANK/U75533