Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplification DNA in a human lung carcinoma cell line

Genes Chromosomes Cancer. 1997 Oct;20(2):208-12. doi: 10.1002/(sici)1098-2264(199710)20:2<208::aid-gcc13>3.0.co;2-m.

Abstract

Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and dmin were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Chromosome Banding
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 10 / genetics*
  • Chromosomes, Human, Pair 7 / genetics
  • Chromosomes, Human, Pair 8 / genetics
  • Chromosomes, Human, Pair 9 / genetics
  • DNA, Neoplasm / analysis
  • Epidermal Growth Factor / genetics
  • Gene Amplification / genetics*
  • Genes, myc / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lung Neoplasms / genetics*
  • Polymerase Chain Reaction
  • Tumor Cells, Cultured

Substances

  • DNA, Neoplasm
  • Epidermal Growth Factor