Lethal outcome of uterine infection in pregnant but not in nonpregnant rats and increased death rate with inhibition of nitric oxide

Am J Reprod Immunol. 1997 Oct;38(4):309-12. doi: 10.1111/j.1600-0897.1997.tb00521.x.

Abstract

Problem: Limited information is available on potential differences in sensitivity to urogenital infections between pregnant and nonpregnant hosts.

Method of study: In this study, we evaluated Escherichia coli infectious complications in pregnant and nonpregnant rats and the effect of nitric oxide (NO) inhibitor, NG-nitro-L-arginine methyl ester (L-NAME), on the outcome of an experimental uterine infection.

Results: Of the infected pregnant animals, 31% were found dead in 24-48 hr. The death rate was increased 2-fold (66%) with L-NAME treatment. No deaths occurred in nonpregnant animals with or without L-NAME treatment. The rate of uterine infection in pregnant animals was about 10-fold higher than in nonpregnant animals.

Conclusion: We propose that infectious complications of pregnancy may be related to gestation-dependent sensitivity to the pathogenic microorganism and the host NO status.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Escherichia coli / pathogenicity
  • Escherichia coli Infections / complications*
  • Escherichia coli Infections / etiology
  • Escherichia coli Infections / metabolism
  • Female
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Nitric Oxide / antagonists & inhibitors*
  • Pregnancy
  • Pregnancy Complications, Infectious / etiology*
  • Pregnancy Complications, Infectious / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Uterine Diseases / complications*
  • Uterine Diseases / etiology
  • Uterine Diseases / metabolism

Substances

  • Nitric Oxide
  • NG-Nitroarginine Methyl Ester