Inhibition of DNA synthesis and tube morphogenesis of cultured vascular endothelial cells by chondromodulin-I

FEBS Lett. 1997 Oct 6;415(3):321-4. doi: 10.1016/s0014-5793(97)01151-4.

Abstract

Cartilage is an avascular tissue, and exhibits anti-angiogenic properties. Cartilage extracts have been shown to contain an inhibitor for DNA synthesis in vascular endothelial cells in vitro. Here we purified the inhibitory activity in the 10-50 kDa fraction of guanidine extracts from fetal bovine epiphyseal cartilage, and found that the inhibitor was identical with chondromodulin-I (ChM-I). Purified ChM-I inhibited tube morphogenesis of cultured vascular endothelial cells, as well as DNA synthesis. These results indicate that cartilage-specific glycoprotein ChM-I may participate in the maintenance of avascularity and anti-angiogenic properties of cartilage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carotid Arteries / cytology
  • Cattle
  • Cell Size / drug effects
  • Cells, Cultured
  • Chromatography, Affinity
  • Chromatography, Gel
  • DNA / biosynthesis*
  • Dose-Response Relationship, Drug
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects*
  • Glycoproteins / metabolism
  • Growth Plate / chemistry*
  • Growth Substances / isolation & purification
  • Growth Substances / metabolism
  • Growth Substances / pharmacology*
  • Intercellular Signaling Peptides and Proteins*
  • Morphogenesis / drug effects
  • Neovascularization, Physiologic / drug effects
  • Nucleic Acid Synthesis Inhibitors / chemistry
  • Nucleic Acid Synthesis Inhibitors / isolation & purification
  • Nucleic Acid Synthesis Inhibitors / pharmacology*
  • Thymidine / metabolism

Substances

  • Glycoproteins
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Nucleic Acid Synthesis Inhibitors
  • DNA
  • Thymidine