Anti-intercellular adhesion molecule-1 (ICAM-1) antibody treatment prevents central and peripheral nervous system disease in autoimmune-prone mice

Lupus. 1997;6(8):645-51. doi: 10.1177/096120339700600805.

Abstract

Abnormal neurological functioning similar to that seen in systemic lupus erythematosus (SLE) patients is detectable in an SLE-prone murine strain (MRL/lpr) by 8-10 weeks and is severe by 18 weeks of age. The purpose of this study was to evaluate the effectiveness of murine antiintercellular adhesion molecule-1 (ICAM-1) in suppressing neurological disease in MRL/lpr mice. Beginning at 6 weeks of age, five MRL/lpr mice received 5 weekly intraperitoneal injections of anti-ICAM-1-containing culture supernatant in phosphate-buffered saline (PBS) whereas four animals were treated with non-anti-ICAM-1 containing supernatant in PBS. A decline in neurological functioning began in control mice by 10 weeks, but anti-ICAM-1 treated mice remained normal throughout the study. All control mice had vasculitic skin lesions by 14 weeks of age whereas none of the anti-ICAM-1 treated mice ever developed skin lesions. Nerve conduction studies performed on all mice prior to sacrifice showed sciatic compound motor action potentials of anti-ICAM-1 treated mice that were of higher amplitude and shorter latency than those of controls. Inflammation in the sciatic nerve was more common in control mice. Brain histology revealed a similar degree of choroid plexus inflammation in both groups. Our study demonstrated that anti-ICAM-1 was effective in suppressing neurological abnormalities in MRL/lpr mice and may potentially be useful therapy in human SLE.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / therapeutic use*
  • Brain Diseases / prevention & control*
  • Female
  • Intercellular Adhesion Molecule-1 / physiology*
  • Lupus Erythematosus, Systemic / complications
  • Lupus Erythematosus, Systemic / therapy*
  • Mice
  • Mice, Inbred MRL lpr
  • Neural Conduction
  • Peripheral Nervous System Diseases / prevention & control*

Substances

  • Antibodies, Monoclonal
  • Intercellular Adhesion Molecule-1