Treatment of a newly established transgenic model of chronic arthritis with nondepleting anti-CD4 monoclonal antibody

J Immunol. 1997 Nov 15;159(10):5032-41.

Abstract

We established a novel animal model for rheumatoid arthritis (RA) by following backcrossing to DBA/1 of (SWR/J x DBA/1)F1 TCR-beta Tg mice, previously reported to be highly susceptible to collagen-induced arthritis. These mice evolved, upon collagen type II immunization, into a chronic arthritis that histopathologically resembles RA. The availability of such a model prompted us to study the role of CD4+ T cells throughout the evolution of disease. Here, we show that administration of nondepleting anti-CD4 not only prevented the evolution of disease but also treated established arthritis. Moreover, functional analyses of T cells isolated from anti-CD4-treated mice demonstrated that the mechanism of protection is not achieved by suppression of the Th1 population but is mediated by induction of collagen type II-specific T cell anergy. Our study suggests that: 1) CD4+ T cells have a fundamental role both in the induction and in the perpetuation of disease; 2) targeting T cells may be an appropriate therapeutic option; and 3) a suitable and well-balanced anti-CD4 treatment may be a valid approach to the control of RA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / therapeutic use*
  • Arthritis, Rheumatoid / genetics
  • Arthritis, Rheumatoid / immunology*
  • Arthritis, Rheumatoid / pathology
  • Arthritis, Rheumatoid / therapy*
  • CD4 Antigens / immunology*
  • Collagen / immunology
  • Collagen / toxicity
  • Cytokines / biosynthesis
  • Disease Models, Animal
  • Disease Susceptibility
  • Epitopes
  • Female
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / blood
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / genetics
  • Male
  • Mice
  • Mice, Inbred DBA
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism

Substances

  • Antibodies, Monoclonal
  • CD4 Antigens
  • Cytokines
  • Epitopes
  • Immunoglobulin G
  • Receptors, Antigen, T-Cell, alpha-beta
  • Collagen