Abstract
mRNA levels of mitogen-activated protein kinase phosphatases, MKP-1 and MKP-2, were determined during chemical hepatocarcinogenesis and during regeneration of rat liver. In chemical hepatocarcinogenesis, the mRNA levels of MKP-1 were increased in primary hepatomas but decreased in rat ascites hepatomas as compared with normal liver. MKP-2 was undetectable in normal liver but strongly expressed in hepatomas. The MKP-2 mRNA level was increased with expression of malignant phenotypes in hepatomas. In regenerating liver, the mRNA level of MKP-1 increased immediately but transiently after partial hepatectomy, and peaked again on day 10, the time when hepatocytes cease proliferation. The elevated expression of MKP-1 on day 10 suggests some roles of MKP-1 as a negative regulator in hepatocyte proliferation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Ascites / enzymology
-
Ascites / genetics
-
Ascites / pathology
-
Carcinoma, Hepatocellular / enzymology
-
Carcinoma, Hepatocellular / genetics*
-
Carcinoma, Hepatocellular / pathology
-
Cell Cycle Proteins*
-
Cell Division / genetics
-
Dual Specificity Phosphatase 1
-
Dual-Specificity Phosphatases
-
Gene Expression Regulation, Neoplastic*
-
Immediate-Early Proteins / biosynthesis
-
Immediate-Early Proteins / genetics*
-
Liver Neoplasms, Experimental / enzymology
-
Liver Neoplasms, Experimental / genetics*
-
Liver Neoplasms, Experimental / pathology
-
Liver Regeneration / genetics*
-
Male
-
Mitogen-Activated Protein Kinase Phosphatases
-
Phosphoprotein Phosphatases*
-
Protein Phosphatase 1
-
Protein Tyrosine Phosphatases / biosynthesis
-
Protein Tyrosine Phosphatases / genetics*
-
RNA, Messenger / biosynthesis
-
Rats
-
Rats, Wistar
Substances
-
Cell Cycle Proteins
-
Immediate-Early Proteins
-
RNA, Messenger
-
Mitogen-Activated Protein Kinase Phosphatases
-
Phosphoprotein Phosphatases
-
Protein Phosphatase 1
-
Dual Specificity Phosphatase 1
-
Dual-Specificity Phosphatases
-
Dusp1 protein, rat
-
Dusp4 protein, rat
-
Protein Tyrosine Phosphatases