Construction and characterization of a recombinant adenovirus directing expression of the MAGE-1 tumor-specific antigen

Int J Cancer. 1997 Sep 17;72(6):1045-55. doi: 10.1002/(sici)1097-0215(19970917)72:6<1045::aid-ijc20>3.0.co;2-2.

Abstract

The finding that many human melanomas express distinct antigens that can be recognised by specific cytolytic T lymphocytes (CTL) implies that immunotherapeutic strategies against this cancer might prove effective. The ex vivo delivery of a tumour-associated antigen to autologous cells and the subsequent re-administration of these cells to the patient might prove effective in boosting the T cell immune response. Recombinant human adenoviral vectors provide an efficient delivery system and have many advantages over other viral and non-viral delivery vehicles. Infection of a panel of human melanoma cell lines by AdCMVMAGE-1, a novel recombinant adenovirus which incorporates the full-length MAGE-1 cDNA, was shown to induce production of high levels of MAGE-1 protein. Incubation of transduced HLA-A1 expressing melanoma cell lines with 2 anti-MAGE-1.A1 CTL clones resulted in specific recognition and lysis of target cells, indicating that the exogenous MAGE-1 protein was processed and presented in a normal manner. Furthermore, quantitative analyses demonstrated a correlation between the efficiency of transduction and the proportion of cells lysed. Importantly for future clinical trials, stimulation of peripheral blood lymphocytes (PBLs) from a melanoma patient by AdCMVMAGE-1-transduced autologous cells resulted in the generation of specific CTLs against the MAGE-1 antigen. Together, our data emphasize the utility of adenoviruses as vaccination vehicles and highlight the potential efficacy of this approach for the treatment of melanoma.

MeSH terms

  • Adenoviridae / genetics*
  • Antigens, Neoplasm / biosynthesis*
  • Cytotoxicity, Immunologic
  • Genetic Vectors
  • Humans
  • Melanoma / immunology*
  • Melanoma-Specific Antigens
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / immunology
  • Recombinant Fusion Proteins / biosynthesis
  • Recombination, Genetic
  • T-Lymphocytes, Cytotoxic / immunology*
  • Transfection / methods
  • Tumor Cells, Cultured
  • beta-Galactosidase / biosynthesis

Substances

  • Antigens, Neoplasm
  • MAGEA1 protein, human
  • Melanoma-Specific Antigens
  • Neoplasm Proteins
  • Recombinant Fusion Proteins
  • beta-Galactosidase